467 Crucial epigenetic modules in skin differentiation

Tissue homeostasis is directed by temporal-spatial regulation of gene expression, where lineage specification a critical step, requiring precise expression profiles. Epigenetic and chromatin accessibility play major role in ensuring adequate transcriptional machinery. We amalgamated data from RNA-Seq, ATAC-Seq, ChIP-Seq HiC to gain transcriptomic/chromatin profile distinct skin epidermal layers. The was generated basal (proliferating) suprabasal (differentiated) cells mouse neonate epidermis. observed repressive signature the layer, processes were enriched repressed genes. This observation supported ATAC-seq analysis. Systematic integration ATAC-Seq RNA-Seq currently being investigated. Given significance Dlx3 differentiation established our lab, we profiled occupancy histones (H3k4me1, H3k4me3, H3k27ac K3k27me3) compartment decipher driving modules. used this delineate super-enhancers suparabsal layer overlay with chromosome conformation HiC-seq. validated subset these modules using Dlx3-knock-out models, marked impaired programs leading significant phenotype. systemic approach will lead comprehensive understanding dynamics crosstalk underlying two strata context Dlx3-mediated higher order regulation, paving way for better management disorders.